The Mouse IL-18 ELISA Kit is a powerful tool for measuring levels of interleukin-18 in mouse serum, plasma, and tissue culture samples. This kit offers superior sensitivity and specificity, providing accurate and consistent results for a variety of research applications.Interleukin-18, also known as IL-18, is a key cytokine involved in the immune response, inflammation, and regulation of cell growth. It plays a critical role in various physiological processes and is implicated in several disease states, including autoimmune disorders, infectious diseases, and inflammatory conditions. By using the Mouse IL-18 ELISA Kit, researchers can gain valuable insights into the role of interleukin-18 in disease pathology and potentially identify new therapeutic targets. With its reliable performance and ease of use, this kit is an essential tool for advancing our understanding of immune regulation and inflammation in mouse models.
Product Name:
Mouse IL-18 ELISA Kit
SKU:
MOES01225
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
