The Mouse IL-9 ELISA Kit is specifically designed for the precise measurement of IL-9 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, guaranteeing accurate and consistent results, which make it perfect for various research purposes.IL-9 is a key cytokine involved in the regulation of immune responses, particularly in allergic and inflammatory conditions. Its dysregulation has been linked to diseases such as asthma, autoimmune disorders, and cancer, highlighting its significance as a biomarker in understanding these conditions and exploring potential treatment options. With the Mouse IL-9 ELISA Kit, researchers can confidently study the role of IL-9 in various disease models and therapeutic interventions, paving the way for new insights and advancements in the field of immunology.
Product Name:
Mouse IL-9 ELISA Kit
SKU:
MOES01219
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
