The Mouse INS (Insulin) ELISA Kit is a specialized assay designed for the quantitative detection of Insulin levels in various biological samples obtained from mice. Insulin is a critical hormone involved in the regulation of blood glucose levels and energy metabolism. It plays a pivotal role in maintaining glucose homeostasis, promoting glucose uptake into cells, and regulating carbohydrate, lipid, and protein metabolism. Accurate measurement of Insulin levels is essential for studying metabolic processes, insulin resistance, diabetes, and related disorders in mouse models. The Mouse INS ELISA Kit from Assay Genie offers exceptional sensitivity, specificity, and precision, ensuring reliable and reproducible results. Manufactured under stringent quality control conditions, this kit provides robust performance and user-friendly protocols, making it an excellent choice for researchers investigating the role of Insulin in metabolic pathways and disease mechanisms using mouse models. Rely on Assay Genie's Mouse INS ELISA Kit for accurate quantification of Insulin levels in your mouse studies.
Product Name:
Mouse INS (Insulin) ELISA Kit
SKU:
AEES00193
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
