The Mouse LDL (Low Density Lipoprotein) ELISA Kit is specifically designed for the precise quantification of low-density lipoprotein levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Low-density lipoprotein, commonly known as "bad cholesterol," is a key player in the development of cardiovascular diseases and atherosclerosis. Monitoring LDL levels in animal models is vital for understanding disease progression and evaluating the efficacy of potential therapeutic interventions. With easy-to-follow instructions and reliable performance, the Mouse LDL ELISA Kit from Assay Genie is an essential tool for researchers studying lipid metabolism, cardiovascular health, and related conditions in mouse models. Order yours today to accelerate your research efforts and advance our understanding of LDL biology.
Product Name:
Mouse LDL (Low Density Lipoprotein) ELISA Kit
SKU:
MOES01674
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
