The Mouse LIF (Leukemia Inhibitory Factor) ELISA Kit is specially designed to accurately measure levels of LIF in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing precise and consistent results for various research purposes.LIF is a key cytokine that plays a critical role in regulating cell growth, differentiation, and survival. It is involved in diverse biological processes, including embryonic development, hematopoiesis, and immune response. Dysregulation of LIF has been linked to various diseases, making it a valuable biomarker for understanding disease mechanisms and identifying potential therapeutic targets.With the Mouse LIF ELISA Kit, researchers can efficiently quantify LIF levels in samples, enabling them to gain insights into the role of LIF in health and disease. This kit is a valuable tool for investigating LIF biology and exploring its potential applications in biomedical research.
Product Name:
Mouse LIF (Leukemia Inhibitory Factor) ELISA Kit
SKU:
MOES00660
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
