The Mouse MT (Melatonin) ELISA Kit is specifically designed for the precise measurement of melatonin levels in mouse serum, plasma, and tissue culture supernatants. With its high sensitivity and specificity, this kit provides dependable and consistent results, making it a valuable tool for various research applications.Melatonin is a critical hormone involved in regulating the circadian rhythm and sleep-wake cycle. It also has antioxidant and anti-inflammatory properties, making it a key player in maintaining overall health and wellness. Studies have shown its potential role in various physiological and pathological processes, including sleep disorders, mood disorders, and neurodegenerative diseases.By accurately measuring melatonin levels with this ELISA kit, researchers can better understand the role of melatonin in different biological processes and potentially develop new treatments or therapies targeting melatonin-related conditions.
Product Name:
Mouse MT (Melatonin) ELISA Kit
SKU:
MOES01266
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
