The Mouse Neutrophil Elastase ELISA Kit is specifically developed for the precise measurement of neutrophil elastase levels in mouse serum, plasma, and cell culture supernatants. Featuring high sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent tool for various research purposes. Neutrophil elastase is a key enzyme secreted by neutrophils, playing a crucial role in inflammatory responses and tissue damage. Dysregulation of neutrophil elastase activity has been linked to various diseases such as acute respiratory distress syndrome, cystic fibrosis, and chronic obstructive pulmonary disease.
Therefore, measuring neutrophil elastase levels can provide valuable insights into pathophysiological processes and potential therapeutic targets for these conditions. The Mouse Neutrophil Elastase ELISA Kit is a valuable resource for researchers studying inflammatory diseases, immune responses, and tissue remodeling processes in mouse models. Its reliable performance and easy-to-use protocol make it a valuable addition to any laboratory conducting experiments related to neutrophil biology and inflammation.
Product Name:
Mouse Neutrophil Elastase ELISA Kit
SKU:
MOFI00993
Reactivity:
Mouse
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1
2
3
Serum (n = 5)
83-101%
90-100%
90-103%
EDTA Plasma (n = 5)
82-100%
87-95%
86-98%
Heparin Plasma (n = 5)
82-101%
80-100%
94-103%
Recovery:
Sample
1
2
Serum (n = 5)
88-103
99
EDTA Plasma (n = 5)
95-104
100
Heparin Plasma (n = 5)
89-100
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
