The Mouse OPG (Osteoprotegerin) ELISA Kit is a reliable tool for the accurate measurement of OPG levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides consistent and precise results, making it suitable for various research purposes. Osteoprotegerin is a key regulator of bone metabolism, acting as a decoy receptor for RANKL and inhibiting osteoclast differentiation and bone resorption. Dysregulation of OPG has been linked to various bone-related diseases, including osteoporosis and bone metastasis, highlighting its importance as a biomarker for studying these conditions and potentially developing therapeutic interventions. Whether investigating bone health, cancer metastasis, or other related research areas, the Mouse OPG ELISA Kit offers a valuable tool for accurate and efficient analysis of Osteoprotegerin levels in mouse samples.
Product Name:
Mouse OPG (Osteoprotegerin) ELISA Kit
SKU:
MOES00685
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
