Mouse OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit
The Assay Genie Mouse OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit is meticulously crafted to facilitate the precise and quantitative measurement of Oxidized Low-Density Lipoprotein (OxLDL) levels within diverse biological samples sourced from mouse models. OxLDL is a pivotal marker associated with oxidative stress, atherosclerosis, and cardiovascular diseases, making accurate monitoring of its concentrations integral to studying lipid metabolism, vascular health, and inflammatory processes. Our Mouse OxLDL ELISA Kit is designed to deliver exceptional sensitivity and specificity, ensuring the generation of reliable and reproducible results crucial for assessing OxLDL's involvement in disease states and potential therapeutic strategies. Manufactured under rigorous quality control measures, this kit ensures robust performance while maintaining user-friendly protocols, making it an optimal choice for research applications focused on investigating the role of Oxidized Low-Density Lipoprotein in mouse models. Trust the Assay Genie Mouse OxLDL ELISA Kit for accurate and dependable quantification of this key biomarker in your experimental investigations.
Product Name:
Mouse OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit
SKU:
AEES00187
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
