Mouse PIIINP (N-Terminal Procollagen III Propeptide) ELISA Kit
The Mouse PIIINP (N-Terminal Procollagen III Propeptide) ELISA Kit is a powerful tool for the quantification of PIIINP levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, delivering reliable and reproducible results for a variety of research applications.PIIINP is a critical marker of collagen synthesis and turnover, serving as a valuable indicator of fibrosis and tissue remodeling processes. By accurately measuring PIIINP levels, researchers can gain insight into various pathological conditions, including liver fibrosis, pulmonary fibrosis, and other connective tissue disorders. With its ease of use and robust performance, the Mouse PIIINP ELISA Kit is an essential asset for studies investigating collagen metabolism and fibrotic diseases in mouse models. Trust in this kit to provide accurate and precise measurements, advancing your research efforts in the field of fibrosis and tissue remodeling.
Product Name:
Mouse PIIINP (N-Terminal Procollagen III Propeptide) ELISA Kit
SKU:
MOES01716
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
