The Mouse Pro-ANP (Pro-Atrial Natriuretic Peptide) CLIA Kit is a cutting-edge multiplex assay designed for the precise quantification of Pro-ANP levels in mouse serum, plasma, and tissue samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an invaluable tool for a diverse range of research applications.Pro-ANP is a key biomarker involved in the regulation of blood pressure and fluid balance, playing a crucial role in cardiovascular health.
Abnormal levels of Pro-ANP have been associated with conditions such as heart failure, hypertension, and kidney disease, making it an essential marker for monitoring and studying these health conditions.With its advanced technology and reliable performance, the Mouse Pro-ANP CLIA Kit is a valuable asset for researchers looking to gain insights into cardiovascular diseases and potential therapeutic interventions.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Pro-ANP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Pro-ANP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Pro-ANP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Pro-ANP. You can calculate the concentration of Mouse Pro-ANP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
96-107
84-96
Average (%)
107
101
91
1:4
Range (%)
89-100
94-108
98-114
Average (%)
94
99
105
1:8
Range (%)
89-103
85-98
102-114
Average (%)
97
92
108
1:16
Range (%)
90-103
101-114
90-105
Average (%)
96
107
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
89-100
95
Cell culture media (n=5)
93-108
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.38
69.71
174.74
28.51
69.35
171.11
Standard deviation
2.98
6.95
13.93
3.23
8.22
10.85
C V (%)
10.88
9.97
7.97
11.33
11.85
6.34
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Pro-ANP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Pro-ANP in samples. No significant cross-reactivity or interference between Mouse Pro-ANP and analogues was observed.
