The Mouse Retinol Binding Protein 4 (RBP4) ELISA Kit is a specialized assay designed to accurately quantify RBP4 levels in various biological samples. RBP4 is a transporter protein that plays a crucial role in the delivery of retinol to tissues and regulates the circulating levels of vitamin A. Dysregulation of RBP4 has been linked to metabolic disorders such as insulin resistance, obesity, and diabetes, making it a valuable biomarker in metabolic research. This ELISA kit enables researchers to investigate the concentrations of RBP4, offering insights into its involvement in metabolic pathways and the development of associated diseases. By accurately measuring RBP4 levels, scientists can better understand the pathophysiological mechanisms underlying metabolic conditions and explore therapeutic strategies aimed at modulating RBP4 expression. The RBP4 ELISA Kit from Assay Genie provides outstanding sensitivity and specificity, ensuring precise and reproducible results. With stringent quality control measures in place, this kit delivers reliable performance and ease of use, making it an ideal choice for cutting-edge research in the field of metabolic disorders and vitamin A metabolism.
Product Name:
Mouse RBP4 (Retinol Binding Protein 4) ELISA Kit
SKU:
AEES00236
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
