The Mouse Serum Amyloid A (SAA) ELISA Kit is a cutting-edge assay designed for the precise quantification of Serum Amyloid A protein levels in a variety of biological samples. Serum Amyloid A is an acute-phase protein primarily produced in response to pro-inflammatory stimuli, making it a crucial biomarker for monitoring inflammatory conditions, infectious diseases, and various pathological processes. Elevations in SAA levels are associated with a spectrum of inflammatory disorders, including rheumatoid arthritis, atherosclerosis, and certain cancers, highlighting its significance in disease diagnosis, prognosis, and treatment evaluation. This ELISA kit empowers researchers and clinicians to accurately measure SAA concentrations, providing valuable insights into the inflammatory status and disease progression. By quantifying SAA levels with precision, scientists can gain a deeper understanding of its role in the acute-phase response and its implications in various inflammatory conditions, enabling tailored therapeutic interventions and personalized patient care. The SAA ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for translational research and clinical applications. Manufactured under stringent quality control standards, this kit delivers robust performance and user-friendly operation, making it the ideal choice for investigating the role of Serum Amyloid A in health and disease pathways with high confidence and accuracy.
Product Name:
Mouse SAA (Serum Amyloid A) ELISA Kit
SKU:
AEES00238
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
75 pg/mL
Detection range:
125-8000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
