The Mouse SELP (P-Selectin) ELISA Kit is specifically designed for the precise measurement of P-Selectin levels in mouse serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, providing accurate and consistent results for various research purposes.P-Selectin is a key adhesion molecule involved in inflammation and immune responses, playing a crucial role in cell adhesion and migration. It is implicated in various pathological conditions such as cardiovascular diseases, inflammatory disorders, and thrombosis, making it a valuable biomarker for studying these diseases and developing novel therapeutic interventions.
Overall, the Mouse SELP (P-Selectin) ELISA Kit is an essential tool for researchers investigating the role of P-Selectin in disease pathogenesis and for identifying potential targets for therapeutic interventions.
Product Name:
Mouse SELP (P-Selectin) ELISA Kit
SKU:
MOES01146
Target:
Mouse SELP (P-Selectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SELP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SELP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SELP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SELP. You can calculate the concentration of Mouse SELP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
98-110
85-100
Average (%)
95
104
92
1:4
Range (%)
93-109
84-97
98-109
Average (%)
100
91
104
1:8
Range (%)
97-112
86-98
96-111
Average (%)
105
91
103
1:16
Range (%)
96-111
87-99
97-111
Average (%)
104
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-109
99
EDTA plasma (n=5)
90-104
95
Cell culture media (n=5)
92-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.3
4.3
0.5
1.2
4.2
Standard deviation
0.03
0.07
0.18
0.03
0.06
0.16
C V (%)
6
5.38
4.19
6.0
5.0
3.81
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse SELP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SELP in samples. No significant cross-reactivity or interference between Mouse SELP and analogues was observed.
