The Mouse Selenoprotein P (SEPP1) ELISA Kit is meticulously developed to facilitate the precise quantification of SEPP1 levels in a variety of biological samples. Selenoprotein P is a crucial plasma glycoprotein that plays a pivotal role in the delivery and regulation of selenium, an essential trace element vital for various physiological processes, including antioxidant defense, thyroid hormone metabolism, and immune response modulation. Perturbations in SEPP1 levels are implicated in conditions related to selenium deficiency, oxidative stress, and metabolic disorders, underscoring its significance as a biomarker in health and disease. This ELISA kit empowers researchers to elucidate the concentrations of SEPP1, offering valuable insights into its functional mechanisms and its implications in diverse health conditions. By accurately measuring SEPP1 levels, scientists can gain a deeper understanding of selenium homeostasis and its impact on overall health, thereby paving the way for targeted interventions and therapeutic strategies to address selenium-related disorders. The SEPP1 ELISA Kit from Assay Genie is engineered with exceptional sensitivity and specificity to deliver robust and reproducible results. With a commitment to stringent quality control standards, this kit ensures reliable performance and user-friendly operation, making it the preferred choice for cutting-edge research exploring the roles of SEPP1 in biological systems and human health.
Product Name:
Mouse SEPP1 (Selenoprotein P) ELISA Kit
SKU:
AEES00237
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
