The Mouse SOST (Sclerostin) ELISA Kit is designed for the precise measurement of SOST levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, providing consistent and accurate results for various research applications.Sclerostin, also known as SOST, is a key regulator of bone metabolism, inhibiting bone formation and promoting bone resorption. Dysregulation of SOST levels has been linked to conditions such as osteoporosis, making it a valuable biomarker for studying bone-related disorders and potential therapeutic interventions. With its reliable performance and ease of use, the Mouse SOST ELISA Kit is an essential tool for researchers investigating the role of SOST in bone health and disease. Get yours today and advance your research with confidence.
Product Name:
Mouse SOST (Sclerostin) ELISA Kit
SKU:
MOES01714
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
