The Mouse IL-12 ELISA Kit - High Sensitivity is an advanced assay designed for the precise and quantitative measurement of Interleukin-12 (IL-12) levels in a variety of biological samples from mouse models. IL-12 is a key cytokine with essential roles in the modulation of immune responses and the regulation of inflammatory reactions, making it a critical component in understanding immune regulation and immune-mediated diseases. This high-sensitivity ELISA kit offers unparalleled accuracy and detection limits, allowing researchers to delve deep into the intricate immune mechanisms involving IL-12. With exceptional sensitivity and specificity, this kit ensures reliable and reproducible results, enabling researchers to explore the immunoregulatory functions of IL-12 in diverse biological contexts. Manufactured under strict quality control measures, the Mouse IL-12 ELISA Kit - High Sensitivity provides robust performance and ease of use, making it an ideal choice for cutting-edge research in immunology, inflammation, and cytokine biology within mouse models.
Product Name:
Mouse IL-12 ELISA Kit (High Sensitivity)
SKU:
AEES00591
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 pg/mL
Detection range:
0.31-20 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
