The Mouse IL-17A ELISA Kit - High Sensitivity is a state-of-the-art assay meticulously crafted to quantitatively measure IL-17A levels in diverse biological samples sourced from mice. IL-17A, a pivotal pro-inflammatory cytokine, serves as a key regulator of immune responses, particularly in the context of promoting inflammation and autoimmune reactions. Its multifaceted roles encompass modulating various cellular functions, immune cell activities, and disease pathogenesis across a spectrum of inflammatory and autoimmune conditions. With a high level of sensitivity, this specialized ELISA kit offers researchers the precise tools needed to investigate the intricate functions and regulatory roles of IL-17A in immune-mediated processes and inflammatory disorders within mouse models. By providing exceptional accuracy and detection capabilities, this high-sensitivity kit empowers researchers to elucidate the complex mechanisms underlying the involvement of IL-17A in immune responses and the development of inflammatory diseases. Maintaining a focus on quality and performance, the Mouse IL-17A ELISA Kit - High Sensitivity from Assay Genie ensures reliable and reproducible results, enabling researchers to conduct in-depth studies with confidence. Designed for ease of use and robust performance, this kit is an excellent choice for exploring IL-17A biology and immune-related research applications in mouse models.
Product Name:
Mouse IL-17A ELISA Kit (High Sensitivity)
SKU:
AEES00593
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
2.19 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
