The Mouse IL-4 ELISA Kit - High Sensitivity is a state-of-the-art assay meticulously developed to enable the quantitative measurement of Interleukin-4 (IL-4) levels across various biological samples obtained from mouse models. IL-4, a crucial cytokine recognized for its diverse roles in modulating immune responses, cellular differentiation, and allergic reactions, plays a pivotal function in orchestrating the body's immune defenses and maintaining immune homeostasis. With exceptional sensitivity, this specialized ELISA kit empowers researchers to delve into the complex intricacies of IL-4-mediated immune responses, inflammatory signaling pathways, and immune cell regulation within mouse models. Providing high sensitivity and precision in detection, this high-sensitivity kit serves as an invaluable tool for investigating the roles of IL-4 in modulating immune function and inflammatory processes. Prioritizing quality and performance, the Mouse IL-4 ELISA Kit - High Sensitivity from Assay Genie ensures the delivery of accurate and reproducible results, facilitating in-depth exploration of IL-4's involvement in immune modulation and disease pathologies. Designed for ease of use and robust performance, this kit is an exceptional choice for researchers conducting studies in immunology, allergy research, and inflammatory diseases utilizing mouse models.
Product Name:
Mouse IL-4 ELISA Kit (High Sensitivity)
SKU:
AEES00595
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
