The Mouse IL-6 ELISA Kit - High Sensitivity is a cutting-edge assay meticulously engineered for the precise quantification of Interleukin-6 (IL-6) levels in various mouse biological samples. IL-6, a pivotal cytokine involved in diverse biological processes including inflammation, immune response modulation, and hematopoiesis, plays a crucial role in the body's defense mechanisms and pathophysiological conditions. This high-sensitivity ELISA kit offers an unparalleled level of accuracy and reliability in IL-6 detection, enabling researchers to unravel the intricate roles of IL-6 in disease mechanisms, autoimmune disorders, cancer, and infections. With exceptional sensitivity and specificity, this kit ensures reproducible results, empowering researchers to explore the complexities of IL-6 biology and pave the way for targeted therapeutic strategies. Manufactured under strict quality control standards, the Mouse IL-6 ELISA Kit - High Sensitivity provides robust performance and user-friendly protocols, making it an indispensable tool for advanced research in immunology, inflammation, and cytokine biology in mouse models.
Product Name:
Mouse IL-6 ELISA Kit (High Sensitivity)
SKU:
AEES00588
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
