The Mouse KIM-1 (Kidney Injury Molecule 1) ELISA Kit - High Sensitivity is a cutting-edge assay meticulously crafted to facilitate the accurate quantification of Kidney Injury Molecule 1 (KIM-1) levels in various biological samples derived from mouse models. KIM-1 is a critical biomarker associated with renal injury and renal pathology, pivotal for the detection and assessment of kidney damage and function, making it a vital target for research into kidney diseases and renal health. Developed with a focus on high sensitivity, this ELISA kit offers researchers a precise tool to delve into the intricate mechanisms of kidney injury, renal function, and related pathologies in mouse models with exceptional accuracy. Through the high-sensitivity detection capabilities of this kit, researchers can gain valuable insights into the role of KIM-1 in kidney injury, inflammation, and disease progression. Ensuring reliable and reproducible results, the Mouse KIM-1 ELISA Kit - High Sensitivity from Assay Genie upholds stringent quality control standards to deliver robust performance. Easy to use and meticulously designed, this kit provides an excellent choice for researchers investigating kidney injury, renal function, and the pathophysiology of kidney diseases in mouse models, enabling advancements in renal research and therapeutic strategies.
Product Name:
Mouse TIM-1/KIM-1 ELISA Kit (High Sensitivity)
SKU:
AEES00596
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
