The Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit - High Sensitivity is a specialized research tool designed for the accurate quantitative analysis of RANTES levels in various biological samples from mouse models. RANTES, a potent chemokine, plays a critical role in regulating immune cell migration, inflammation, and immune responses. This high-sensitivity ELISA kit empowers researchers to precisely measure RANTES concentration, providing valuable insights into its involvement in inflammatory processes, immune modulation, and disease pathogenesis within the mouse model context. Accurate quantification of RANTES is essential for advancing the understanding of its functions in immune regulation and inflammatory diseases. Manufactured to meet rigorous quality control standards, the Mouse RANTES ELISA Kit - High Sensitivity offered by Assay Genie ensures dependable and reproducible results, making it an ideal choice for researchers seeking robust performance and ease of use in their investigative studies.
Product Name:
Mouse RANTES/CCL5 ELISA Kit (High Sensitivity)
SKU:
AEES00614
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
