The Mouse VEGF-A (Vascular Endothelial Growth Factor A) ELISA Kit - High Sensitivity is a cutting-edge assay meticulously developed for the precise and quantitative measurement of VEGF-A levels in various biological samples from mouse models. VEGF-A, a vital pro-angiogenic factor, plays a crucial role in the regulation of blood vessel growth and endothelial cell function, impacting various physiological and pathological processes such as angiogenesis, vascular permeability, and tissue repair. This high-sensitivity ELISA kit offers exceptional accuracy and detection limits, enabling researchers to explore the intricate roles of VEGF-A in vascular development, tumor angiogenesis, and various vascular-related disorders with enhanced precision. With unparalleled sensitivity and specificity, this kit ensures reliable and reproducible results, empowering researchers to delve deep into the mechanisms involving VEGF-A in diverse biological contexts. Manufactured under stringent quality control standards, the Mouse VEGF-A ELISA Kit - High Sensitivity provides robust performance and user-friendly protocols, making it an ideal choice for advanced research in vascular biology, angiogenesis, and vascular-targeted therapies within mouse models.
Product Name:
Mouse VEGF-A ELISA Kit (High Sensitivity)
SKU:
AEES00592
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
3.21 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
