NCC Antibody is a rabbit polyclonal antibody directed against NCC, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF and IEM. Reported reactivity: Human, Mouse, Rat and Dog. Purified by Affinity.
The Na⁺-Cl⁻ co-transporter (NCC), primarily expressed in the distal convoluted tubule of the kidney, is a thiazide-sensitive transporter critical for sodium and chloride reabsorption. While traditionally studied in renal physiology and hypertension, emerging evidence suggests that NCC may also play a role in neurodegenerative disease through its influence on systemic electrolyte balance and neuronal excitability. NCC is activated by phosphorylation and regulated by signaling pathways involving SGK1 and WNK kinases. Dysregulation of NCC activity can alter extracellular potassium and sodium levels, indirectly affecting neuronal membrane potential and synaptic transmission. Additionally, NCC expression has been detected in non-renal tissues, including the lens and possibly the brain, suggesting broader physiological roles. In neurodegenerative contexts, electrolyte imbalance and altered ion transport are known contributors to neuronal dysfunction and glial activation. NCC-mediated sodium handling may influence neuroinflammatory responses and oxidative stress, particularly in conditions such as Alzheimer’s disease and vascular dementia. Furthermore, thiazide diuretics targeting NCC have been associated with cognitive effects, underscoring the transporter’s potential relevance in brain health. Understanding NCC’s extrarenal functions may reveal novel mechanisms linking ion transport to neurodegeneration and offer new therapeutic avenues for modulating neuronal homeostasis. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
Product Name:
NCC Antibody
Product SKU:
STPA00046
Size:
100 µg
Target:
NCC
Host Species:
Rabbit
Antibody Type:
Polyclonal
Research Areas:
Cancer, Neuroscience, Pumps/Transporters, Ion Pumps/Transporters
Buffer:
PBS, 50% glycerol, 0.09% sodium azide
Storage & Shipping:
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:
Liquid
Applications:
WB, IHC, ICC/IF, IEM
Purification:
Affinity Purified
Concentration:
1 mg/ml
Reactivity:
Human, Mouse, Rat, Dog
Recommended Dilution:
WB (1:1000), IHC (1:200); optimal dilutions for assays should be determined by the user.
Specificity:
Detects ~160kDa.
Guarantee:
12 months from date of dispatch
Immunogen:
Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
Immunogen Species:
Rat
Accession Number:
NP_062218, NP_000330
Gene ID:
54300, 6559
Swiss-Prot:
P55018, P55017
Cellular Localization:
Membrane
Alternative Names:
NCC, SLC12A3, SCYL1, CKb10, MCP-4, MGC17134, NCC-1, NCC1, SCYA13, CK-beta-10, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, New CC chemokine 1, Small inducible cytokine A13, Small inducible cytokine subfamily A (Cys-Cys) member 13, Chemokine (C-C)
References:
1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712. 2. Tiwari S., et al. (2009) Am J Nephrol. 30(6): 554-562. 3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.
