The PGD2 (Prostaglandin D2) ELISA Kit is specifically designed for the precise measurement of PGD2 levels in biological samples such as serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for various research applications.Prostaglandin D2 is a lipid mediator that plays a key role in inflammatory responses, allergic reactions, and immune regulation. Abnormal levels of PGD2 have been linked to various diseases such as asthma, allergic rhinitis, and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions. With its advanced technology and user-friendly protocols, the PGD2 ELISA Kit from Assay Genie is a valuable tool for researchers looking to explore the role of PGD2 in health and disease. Get accurate and reliable data with this innovative ELISA kit.
Product Name:
PGD2 (Prostaglandin D2) ELISA Kit
SKU:
UNES00043
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
