Phospho-CBL-Y731 Rabbit Polyclonal Antibody (CABP1073)

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SKU:
CABP1073
€99 - €319

Description

Technical Manual

Phospho-CBL-Y731 Rabbit Polyclonal Antibody

Product Name:Phospho-CBL-Y731 Rabbit Polyclonal Antibody
Product SKU:CABP1073
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Jul 2016]

Immunogen:A phospho synthetic peptide corresponding to residues surrounding Y731 of human CBL.
Sequence:

Email for sequence

Gene ID:867
Swiss Prot:P22681
Synonyms:C-CBL; CBL2; FRA11B; NSLL; RNF55; CBL; c-Cbl
Calculated MW:99kDa
Observed MW:Refer to figures
Reactivity:Human
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting 
Positive Samples:
Cellular Location:Cell membrane, Cytoplasm

Uniprot Information

UniProt Protein Function:Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.
NCBI Summary:This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Jul 2016]
UniProt Code:P22681
NCBI GenInfo Identifier:251757253
NCBI Gene ID:867
NCBI Accession:P22681.2
UniProt Secondary Accession:P22681,A3KMP8,
UniProt Related Accession:P22681
Molecular Weight:99,633 Da
NCBI Full Name:E3 ubiquitin-protein ligase CBL
NCBI Synonym Full Names:Cbl proto-oncogene
NCBI Official Symbol:CBL 
NCBI Official Synonym Symbols:CBL2; NSLL; C-CBL; RNF55; FRA11B 
NCBI Protein Information:E3 ubiquitin-protein ligase CBL
UniProt Protein Name:E3 ubiquitin-protein ligase CBL
UniProt Synonym Protein Names:Casitas B-lineage lymphoma proto-oncogene; Proto-oncogene c-Cbl; RING finger protein 55; RING-type E3 ubiquitin transferase CBLCurated; Signal transduction protein CBL
Protein Family:E3 ubiquitin ligase
UniProt Gene Name:CBL 
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Additional Information

Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
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