The Phospho-CDK9-T186 Antibody (CABP0810) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically recognizes the phosphorylated form of CDK9 at threonine 186, a critical residue involved in the control of transcriptional elongation.CDK9 is a key regulator of gene expression and plays a crucial role in various cellular processes, including cell cycle progression and transcriptional regulation. Phosphorylation of CDK9 at T186 is known to affect its kinase activity and substrate specificity, making it an important target for investigating the molecular mechanisms underlying transcriptional regulation.The Phospho-CDK9 (T186) Polyclonal Antibody is extensively validated for use in Western blot applications with human samples, providing researchers with a reliable tool for detecting and quantifying phospho-CDK9 levels in various biological samples.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-CDK9-T186 Antibody
SKU:
CABP0810
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
RYTN R
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TAK, C-2k, CTK1, CDC2L4, PITALRE, Phospho-CDK9-T186
Positive Sample:
NIH/3T3
Cellular Localization:
Cytoplasm, Nucleus, Pml Body.
Calculated MW:
43kDa
Observed MW:
43kDa
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
Purification Method
Affinity purification
Gene ID
1025
RRID
AB_2770984
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK9-T186 Rabbit pAb (CABP0810) at 1:1000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
