The Phospho-C/EBPB-T235 Antibody (CABP1055) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically detects the phosphorylated form of C/EBPβ at threonine 235 in human samples and is validated for use in Western blot applications. C/EBPβ is a key regulator of gene expression involved in various cellular processes, including differentiation, proliferation, and immune response. Phosphorylation at threonine 235 is known to impact the transcriptional activity of C/EBPβ and regulate its function in different cellular contexts.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-C/EBPB-T235 Antibody
SKU:
CABP1055
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PGTP S
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions.
Purification Method
Affinity purification
Gene ID
1051
RRID
AB_2863927
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-C/EBPB-T235 Rabbit pAb (CABP1055) at 1:1000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. NIH/3T3 cells were treated by Serum-starvation overnight at 37℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
