Anti-Phospho-PPP1R12A-T696 Antibody (CABP0915)

SKU:
CABP0915
€99 - €319

Description

Technical Manual

Anti-Phospho-PPP1R12A-T696 Antibody

Product Name:Anti-Phospho-PPP1R12A-T696 Antibody
Product SKU:CABP0915
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen:A synthetic phosphorylated peptide around T696 of human PPP1R12A.
Sequence:

Email for sequence

Gene ID:4659
Swiss Prot:O14974
Synonyms:PPP1R12A; M130; MBS; MYPT1; MYPT1
Calculated MW:105kDa/109kDa/111kDa/115kDa
Observed MW:Refer to figures
Reactivity:Human
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:
Cellular Location:Cytoplasm

Uniprot Information

UniProt Protein Function:MYPT1: myosin phosphatase target subunit 1, a regulatory subunit of protein phosphatase 1. Also called the myosin-binding subunit of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. Four splice-variant isoforms have been described.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 12q15-q21

Cellular Component: A band; actin cytoskeleton; centrosome; contractile fiber; cytoplasm; cytosol; focal adhesion; kinetochore; nucleoplasm; Z disc

Molecular Function:enzyme inhibitor activity; phosphatase regulator activity; phosphoprotein phosphatase activity; protein binding; protein kinase binding; signal transducer activity

Biological Process: centrosome organization and biogenesis; G2/M transition of mitotic cell cycle; mitosis; mitotic cell cycle; negative regulation of catalytic activity; positive regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; regulation of cell adhesion; regulation of nucleocytoplasmic transport; small GTPase mediated signal transduction

NCBI Summary:Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]
UniProt Code:O14974
NCBI GenInfo Identifier:41017262
NCBI Gene ID:4659
NCBI Accession:O14974.1
UniProt Secondary Accession:O14974,Q2NKL4, Q569H0, Q86WU3, Q8NFR6, Q9BYH0, B4DZ09 F8VWB4,
UniProt Related Accession:O14974
Molecular Weight:105,641 Da
NCBI Full Name:Protein phosphatase 1 regulatory subunit 12A
NCBI Synonym Full Names:protein phosphatase 1 regulatory subunit 12A
NCBI Official Symbol:PPP1R12A
NCBI Official Synonym Symbols:MBS; M130; MYPT1
NCBI Protein Information:protein phosphatase 1 regulatory subunit 12A
UniProt Protein Name:Protein phosphatase 1 regulatory subunit 12A
UniProt Synonym Protein Names:Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit
Protein Family:Protein phosphatase 1 regulatory
UniProt Gene Name:PPP1R12A
UniProt Entry Name:MYPT1_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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