Anti-Phospho-STAT5A-Y694 Antibody (CABP0758)

SKU:
CABP0758
€129 - €389

Description

Technical Manual

Anti-Phospho-STAT5A-Y694 Antibody

Product Name:Anti-Phospho-STAT5A-Y694 Antibody
Product SKU:CABP0758
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]

Immunogen:A synthesized peptide derived from human Phospho-Stat5 (Y694).
Sequence:

Email for sequence

Gene ID:6776
Swiss Prot:P42229
Synonyms:MGF; STAT5; STAT5A
Calculated MW:90kDa
Observed MW:100KDa
Reactivity:Human, Mouse, Rat
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry 
Positive Samples:TF-1
Cellular Location:

Product Images

Anti-Phospho-STAT5A-Y694 Antibody (CABP0758)

Western blot - Phospho-STAT5A-Y694 Rabbit mAb (CABP0758)

Uniprot Information

UniProt Protein Function:STAT5A: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 fusion protein is essential for the tumorigenesis. Induces the expression of BCL2L1/BCL-X(L) in the mouse, suggesting an antiapoptotic function of this protein. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor; Motility/polarity/chemotaxis; Oncoprotein

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; cytosol

Molecular Function:signal transducer activity; protein binding; transcription factor activity

Biological Process: lactation; succinate metabolic process; oxaloacetate metabolic process; peptidyl-tyrosine phosphorylation; positive regulation of interleukin-2 biosynthetic process; T cell differentiation in the thymus; positive regulation of multicellular organism growth; female pregnancy; positive regulation of mitotic cell cycle; fatty acid metabolic process; positive regulation of activated T cell proliferation; 2-oxoglutarate metabolic process; positive regulation of natural killer cell differentiation; sequestering of lipid; natural killer cell differentiation; allantoin metabolic process; negative regulation of mast cell apoptosis; luteinization; development of secondary male sexual characteristics; regulation of steroid metabolic process; creatinine metabolic process; T cell homeostasis; positive regulation of gamma-delta T cell differentiation; Peyer's patch development; isoleucine metabolic process; negative regulation of erythrocyte differentiation; transcription, DNA-dependent; regulation of multicellular organism growth; citrate metabolic process; valine metabolic process; development of secondary female sexual characteristics; positive regulation of natural killer cell mediated cytotoxicity; JAK-STAT cascade; creatine metabolic process; regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell differentiation; positive regulation of B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; taurine metabolic process; positive regulation of inflammatory response

NCBI Summary:The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]
UniProt Code:P42229
NCBI GenInfo Identifier:1174462
NCBI Gene ID:6776
NCBI Accession:P42229.1
UniProt Secondary Accession:P42229,Q1KLZ6,
UniProt Related Accession:P42229
Molecular Weight:
NCBI Full Name:Signal transducer and activator of transcription 5A
NCBI Synonym Full Names:signal transducer and activator of transcription 5A
NCBI Official Symbol:STAT5A 
NCBI Official Synonym Symbols:MGF; STAT5 
NCBI Protein Information:signal transducer and activator of transcription 5A
UniProt Protein Name:Signal transducer and activator of transcription 5A
Protein Family:Signal transducer and activator of transcription
UniProt Gene Name:STAT5A 
UniProt Entry Name:STA5A_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
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