The Phospho-VEGF Receptor 2-Y1175 Antibody (CABP1095) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. VEGFR2 plays a key role in angiogenesis, the process by which new blood vessels form from existing vessels. Phosphorylation of tyrosine 1175 on VEGFR2 is known to be crucial for its activation and downstream signaling, making it a critical target for investigation in cancer research, cardiovascular diseases, and other conditions involving abnormal blood vessel growth.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-VEGF Receptor 2-Y1175 Antibody
SKU:
CABP1095
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cell Junction, Cell Membrane, Cytoplasm, Cytoplasmic Vesicle, Early Endosome, Endoplasmic Reticulum, Nucleus, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
152kDa
Observed MW:
230kDa
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
Purification Method
Affinity purification
Gene ID
3791
RRID
AB_2863964
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-VEGF Receptor 2-Y1175 pAb (CABP1095) at 1:1000 dilution. HUVEC cells were treated with VEGF (100 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
