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Phosphorylation ELISA Kits

Phosphorylation ELISA Kits | 69 Phospho-Site Kits | Assay Genie
ELISA Kits · Phosphorylation

Measure phosphorylation without the western blot

Quantitative and cell-based sandwich ELISA kits that verify pathway activation straight from cell lysates — phosphorylated protein and pan protein, measured side by side.

No western blotPhospho + pan side-by-sideRead at 450 nm
Browse all kitsTalk to our PhD team
Akt1 (Phospho-Ser473) Colorimetric Cell-Based ELISA Kit
AKT (p-Ser473)
STAT3 (Phospho-Tyr705) Colorimetric Cell-Based ELISA Kit
STAT3 (p-Tyr705)
p53 (Phospho-Ser15) Colorimetric Cell-Based ELISA Kit
p53 (p-Ser15)
69
Phospho kits
12
Signalling pathways
Phospho + pan
Measured together
450 nm
Colorimetric read
How the assay works

A sandwich ELISA, end to end in four steps

A capture antibody immobilises target protein from your lysate; a phospho-specific detection antibody and HRP secondary generate colour with TMB, read at 450 nm in proportion to bound target.

1

Coat & capture

Plate-bound capture antibody binds your target from lysate.

2

Wash

Unbound material is washed from the wells.

3

Detect

Phospho-specific antibody + HRP secondary bind the target.

4

Read at 450 nm

TMB develops; stop solution turns blue to yellow. Signal ∝ target.

Featured kits

Popular phospho-site kits

Cell-based kits that quantify phosphorylated and total protein in the same assay, normalised to cell number.

PI3K/AKT/mTORAkt1 (Phospho-Ser473) Colorimetric Cell-Based ELISA Kit

Akt1 (Phospho-Ser473) Colorimetric Cell-Based ELISA Kit

mTORC2 site; PI3K/AKT pathway activation.

View kit →
JAK/STATSTAT3 (Phospho-Tyr705) Colorimetric Cell-Based ELISA Kit

STAT3 (Phospho-Tyr705) Colorimetric Cell-Based ELISA Kit

Cytokine-driven STAT3 activation marker.

View kit →
DNA Damagep53 (Phospho-Ser15) Colorimetric Cell-Based ELISA Kit

p53 (Phospho-Ser15) Colorimetric Cell-Based ELISA Kit

ATM-dependent DNA-damage response site.

View kit →
Find your kit

All 69 phosphorylation kits

Grouped by signalling pathway — every link verified against the current catalogue. Filter by pathway or search by target, site or format.

All pathwaysPI3K/AKT/mTORMAPKJAK/STATRTKSFK & ImmuneDNA DamageAMPK & MetabolicNF-κBApoptosis & AutophagyEpigeneticNeuroOther
TargetSiteReactivityPathwayFormat
4E-BP1T36HMPI3K/AKT/mTORCell-BasedView →
AKTS473HMRPI3K/AKT/mTORQuantitativeView →
AKTS473HMRPI3K/AKT/mTORCell-BasedView →
GSK3αS21HMRPI3K/AKT/mTORQuantitativeView →
GSK3αS21HMRPI3K/AKT/mTORCell-BasedView →
GSK3βS9HMRPI3K/AKT/mTORQuantitativeView →
GSK3βS9HMRPI3K/AKT/mTORCell-BasedView →
mTORS2448HMPI3K/AKT/mTORCell-BasedView →
PDK1Ser241HMRPI3K/AKT/mTORCell-BasedView →
PTENS380HMRPI3K/AKT/mTORCell-BasedView →
RPS6S235/S236HMRPI3K/AKT/mTORQuantitativeView →
c-FosThr232HMAPKCell-BasedView →
c-JunS63HMRMAPKCell-BasedView →
CREBS133HMAPKCell-BasedView →
JNKT183/Y185HMRMAPKCell-BasedView →
MKK3S189HMRMAPKCell-BasedView →
MKK6S207HMRMAPKCell-BasedView →
MSK1S376HMAPKCell-BasedView →
PAK2Ser20HMAPKCell-BasedView →
RSK1S380HMAPKCell-BasedView →
JAK1Y1022HMRJAK/STATCell-BasedView →
JAK2Y221HJAK/STATCell-BasedView →
STAT1S727HMJAK/STATCell-BasedView →
STAT1Y701HMJAK/STATCell-BasedView →
STAT3Y705HMRJAK/STATCell-BasedView →
STAT4Y693HJAK/STATCell-BasedView →
STAT5AY694HJAK/STATCell-BasedView →
STAT6Y641HJAK/STATCell-BasedView →
Caveolin-1Tyr14HRTKCell-BasedView →
EGFRS1070HRTKCell-BasedView →
EGFRY1172HMRTKCell-BasedView →
EphB1Y594/Y604HRTKCell-BasedView →
FGFR1Y654HRTKCell-BasedView →
FRS2Y436HMRRTKCell-BasedView →
IGF1RY1165/1166HRTKCell-BasedView →
METY1234HMRRTKCell-BasedView →
PDGFRAY849HRTKCell-BasedView →
RETY1062HRTKCell-BasedView →
TIE2Y1108HRTKCell-BasedView →
VEGFR2Y951HRTKCell-BasedView →
BTKY223HSFK & ImmuneCell-BasedView →
BTKY551HSFK & ImmuneCell-BasedView →
c-AblY245HSFK & ImmuneCell-BasedView →
FERY402HSFK & ImmuneCell-BasedView →
FYNY530HSFK & ImmuneCell-BasedView →
LCKY505HSFK & ImmuneCell-BasedView →
PLCγ1Tyr771HMRSFK & ImmuneCell-BasedView →
PLCγ2Tyr753HMSFK & ImmuneCell-BasedView →
PYK2Y881HSFK & ImmuneCell-BasedView →
SHP2Y542HMRSFK & ImmuneQuantitativeView →
SHP2Y542HMRSFK & ImmuneCell-BasedView →
SYKY525HSFK & ImmuneCell-BasedView →
ZAP70Y493HSFK & ImmuneCell-BasedView →
Chk1S280HDNA DamageCell-BasedView →
Chk2T68HDNA DamageCell-BasedView →
Cyclin B1S126HDNA DamageCell-BasedView →
H2AXS139HDNA DamageCell-BasedView →
HSP27S82HDNA DamageCell-BasedView →
NBS1S343HDNA DamageCell-BasedView →
p27/Kip1T198HDNA DamageCell-BasedView →
p53S15HMRDNA DamageCell-BasedView →
LKB1Ser428HAMPK & MetabolicCell-BasedView →
IκBαS32/S36HNF-κBCell-BasedView →
NF-κB p65S536HMRNF-κBCell-BasedView →
BADS112HApoptosis & AutophagyIn-CellView →
HDAC4S632HMREpigeneticCell-BasedView →
α-synucleinY133HNeuroCell-BasedView →
TauT181HMRNeuroCell-BasedView →
IRF-3S386HOtherCell-BasedView →

Don’t see your target or site?

Our PhD-level team can develop a custom phospho ELISA for your target, or point you to the right assay format.

Talk to our team
69 phospho kits12 pathwaysNo western blotPhD-level technical support