The Porcine CRP (C-Reactive Protein) ELISA Kit serves as a specialized assay designed for the precise and quantitative measurement of C-Reactive Protein (CRP) levels in various biological samples sourced from porcine models. CRP is a key biomarker of inflammation, extensively involved in numerous cellular functions such as immune response modulation, cell differentiation, and associated cascades of biological pathways. Its role in acute-phase responses and inflammatory conditions underscores its utility in understanding disease progression, immune processes, and responses to infections in porcine systems. With a commitment to delivering accurate results, the Porcine CRP ELISA Kit provided by Assay Genie offers unparalleled sensitivity and specificity, ensuring the reproducibility and dependability of experimental outcomes. Manufactured under strict quality control measures, this ELISA kit is engineered to deliver consistent and robust performance, making it an ideal choice for researchers conducting studies related to porcine inflammatory responses, immune system dynamics, and disease pathology. Its user-friendly design further enhances its suitability for a wide range of research applications, empowering scientists with a valuable tool to explore the role of CRP in porcine health and disease states.
Product Name:
Porcine CRP (C-Reactive Protein) ELISA Kit
SKU:
AEES00274
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.