The Porcine PP (Pepsin) ELISA Kit is a specialized assay crafted for the precise measurement of Pepsin levels in porcine biological samples. Pepsin, an essential enzyme in gastric physiology, plays a crucial role in the breakdown of proteins into peptides during digestion, particularly in the stomach of pigs. Monitoring Pepsin concentrations enables researchers to assess gastric function, digestive health, and nutrient absorption in porcine models. This ELISA kit offers a reliable and accurate method for quantifying Pepsin levels, providing valuable insights into porcine digestive processes and gastric health. By measuring Pepsin concentrations, researchers can evaluate the enzymatic activity in the stomach, investigate digestive disorders, and explore dietary influences on the digestive system of pigs. With exceptional sensitivity and specificity, the Porcine PP ELISA Kit ensures precise and reproducible results, facilitating in-depth studies on porcine gastrointestinal function and nutritional metabolism. Manufactured under strict quality control standards, this kit delivers robust performance and user-friendly protocols, making it an indispensable tool for research aimed at understanding porcine digestive physiology and dietary impact.
Product Name:
Porcine PP (Pepsin) ELISA Kit
SKU:
AEES00691
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 ng/mL
Detection range:
78.13-5000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
