The QuickStep Canine E3 Estriol ELISA Kit is a cutting-edge assay designed for the precise measurement of E3 estriol levels in canine serum, plasma, and tissue samples. This kit offers exceptional sensitivity and accuracy, ensuring dependable results for a variety of research and clinical applications.E3 estriol is a key hormone involved in canine reproductive health and pregnancy monitoring. Monitoring E3 estriol levels can provide valuable insights into the reproductive status of female dogs, aiding in fertility management and monitoring of pregnancy complications.
With its high-quality components and user-friendly protocol, the QuickStep Canine E3 Estriol ELISA Kit is an indispensable tool for researchers and veterinarians interested in canine reproductive biology and health. Don't miss out on this advanced kit for your research needs.
Product Name:
QuickStep Canine Estriol ELISA Kit
Product Code:
QSES008
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Canine
Sensitivity:
1.17 pg/mL
Range:
3.13-200 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Estriol
Application:
This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.
Specificity:
This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Canine serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Canine E3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Canine E3 are added to the micro ELISA plate wells. Canine E3 in samples (or standards) competes with a fixed amount of Canine E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to Canine E3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Canine E3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
9.40
24.67
83.80
10.26
23.44
79.70
Standard deviation
0.48
1.06
3.13
0.56
1.13
3.58
CV (%)
5.07
4.28
3.73
5.43
4.83
4.49
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
86-101
93
EDTA plasma(n=8)
96-107
101
Samples were spiked with high concentrations of Canine Pg and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
