The QuickStep Human IL-6 (Interleukin-6) ELISA Kit is specifically designed for the accurate and sensitive detection of IL-6 levels in human serum, plasma, and cell culture supernatants. This kit offers high specificity and sensitivity, providing reliable and reproducible results for a variety of research applications.IL-6 is a key cytokine involved in inflammation and immune response, playing a critical role in various diseases including autoimmune disorders, cancer, and infectious diseases. Monitoring IL-6 levels can provide valuable insights into disease progression and response to treatment, making it a valuable biomarker for research and clinical studies.
With the QuickStep Human IL-6 ELISA Kit, researchers can accurately measure IL-6 levels in human samples, enabling further understanding of the role of this cytokine in different disease conditions and potential therapeutic interventions. This kit is easy to use, offering a convenient and efficient solution for studying IL-6 levels in various research settings.
Product Name:
QuickStep Human IL-6 ELISA Kit
Product Code:
QSES015
Assay Type:
Sandwich
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Human
Sensitivity:
1.02 pg/mL
Range:
3.13-200 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
IL-6
Application:
This ELISA kit applies to the in vitro quantitative determination of IL-6 concentrations in serum and plasma.
Specificity:
This kit recognizes IL-6 in samples. No significant cross-reactivity or interference between IL-6 and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Human serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IL-6 are added to the micro ELISA plate wells. Human IL-6 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IL-6. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human IL-6. The concentration of Human IL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
9.10
22.29
90.92
9.12
21.98
90.28
Standard deviation
0.57
1.13
3.32
0.53
1.31
3.25
CV (%)
6.24
5.06
3.65
5.84
5.98
3.60
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
89-102
96
EDTA plasma(n=8)
92-105
97
Samples were spiked with high concentrations of Human IL-6 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
