Rat AGER (Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit
The Rat AGER (Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit is a specialized assay developed for the quantitative detection of AGER levels in various rat biological samples. AGER, also known as RAGE (Receptor for Advanced Glycation End Products), is a multi-ligand receptor involved in diverse cellular processes, including inflammation, immune response, and cell proliferation. AGER plays a crucial role in initiating cellular signaling pathways in response to advanced glycation end products, which contribute to various pathological conditions. Accurate measurement of AGER is essential for understanding its role in diabetic complications, neurodegenerative diseases, cancer, and cardiovascular disorders. The Rat AGER ELISA Kit enables researchers to study the expression and function of AGER in rat models, providing insights into the mechanisms underlying disease pathogenesis and the development of potential therapeutic strategies. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, the kit provides reliable performance and user-friendly protocols, making it an excellent choice for research aimed at elucidating the functions of AGER and its implications in various diseases.
Product Name:
Rat AGER (Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit
SKU:
AEES00489
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
