The Rat AGRN (Agrin) ELISA Kit is specifically designed for precise and reliable detection of agrin levels in rat serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reproducible results that are essential for a variety of research applications.Agrin is a key protein that plays a critical role in synaptic plasticity and development, making it a valuable biomarker for studying neurological disorders, neuromuscular diseases, and other conditions affecting the nervous system. By accurately measuring agrin levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic opportunities.With its robust performance and easy-to-use format, the Rat AGRN (Agrin) ELISA Kit is a valuable tool for researchers looking to study agrin and its role in various biological processes. Get reliable and precise results with this high-quality ELISA kit from Assay Genie.
Product Name:
Rat AGRN (Agrin) ELISA Kit
SKU:
RTES01016
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.