The Rat ANG II (Angiotensin II) ELISA Kit is specially designed for the accurate measurement of angiotensin II levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Angiotensin II is a key hormone involved in the regulation of blood pressure and fluid balance, playing a crucial role in the renin-angiotensin system. Dysregulation of angiotensin II levels has been linked to various cardiovascular diseases, renal disorders, and hypertension, making it a valuable biomarker for understanding these conditions and potential therapeutic interventions. With its reliable performance and ease of use, the Rat ANG II ELISA Kit is an essential tool for researchers studying the physiological and pathological roles of angiotensin II in animal models. Get accurate and reliable data with this advanced assay kit from Assay Genie.
Product Name:
Rat ANG II (Angiotensin II) ELISA Kit
SKU:
RTES01000
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
