Rat BDNF (Brain Derived Neurotrophic Factor) ELISA Kit
The Rat BDNF (Brain-Derived Neurotrophic Factor) ELISA Kit is specifically designed for the quantitative detection of BDNF levels in rat samples, including serum, plasma, and tissue homogenates. This ELISA kit offers exceptional sensitivity and specificity, delivering accurate and reproducible results for various research applications.BDNF is a key neurotrophic factor that supports the growth, survival, and function of neurons in the brain. It is involved in numerous physiological processes, including neuroplasticity, learning, and memory. Dysregulation of BDNF expression has been linked to various neurological disorders, such as Alzheimer's disease, depression, and schizophrenia, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Rat BDNF ELISA Kit provides researchers with a powerful tool to investigate the role of BDNF in neurobiology and uncover novel insights into neurological diseases.
Product Name:
Rat BDNF (Brain Derived Neurotrophic Factor) ELISA Kit
SKU:
RTES00974
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
