The Rat C4 (Complement Component 4) ELISA Kit is a specialized assay designed to accurately quantify the levels of Complement Component 4 in various biological samples obtained from rat models. Complement Component 4 plays a critical role in the complement system, a fundamental part of the immune response responsible for enhancing immune defenses, eliminating pathogens, and promoting inflammation. Measurement of C4 levels is essential for evaluating the activation status of the complement pathway, immune responses, and inflammatory processes. By utilizing this ELISA kit, researchers can investigate the dynamics of C4 levels in different biological contexts, shedding light on its involvement in immune regulation, infectious diseases, autoimmune disorders, and inflammatory conditions. Understanding the quantitative levels of Complement Component 4 is crucial for unraveling its contribution to host defense mechanisms and immune-mediated diseases, providing insights into potential therapeutic interventions and disease management strategies. The Rat C4 ELISA Kit offered by Assay Genie ensures exceptional sensitivity and specificity, enabling precise and reliable quantification of C4 levels in rat samples. Manufactured with stringent quality control measures, this kit delivers robust and reproducible performance, making it a valuable tool for research endeavors exploring the complex interplay of the complement system and immune responses in various disease states.
Product Name:
Rat C4 (Complement Component 4) ELISA Kit
SKU:
AEES00363
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
