The Rat CASP9 (Caspase 9) ELISA Kit is a specialized assay designed for the quantitative detection of Caspase 9 levels in rat biological samples. Caspase 9 is a critical enzyme involved in programmed cell death, also known as apoptosis, playing a key role in regulating cell survival and proliferation. Accurate measurement of Caspase 9 levels is essential for understanding its role in apoptotic pathways, regulatory mechanisms in cell death, and its implications in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. The ELISA kit provides a reliable and precise method to quantify Caspase 9 concentrations in biological samples, enabling researchers to investigate its involvement in various cellular processes and disease states. Manufactured under stringent quality control standards, the Rat CASP9 ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Its user-friendly protocols, robust performance, and accurate measurements make it a valuable tool for researchers studying apoptosis, cell signaling pathways, and therapeutic strategies targeting Caspase 9 in various research fields.
Product Name:
Rat CASP9 (Caspase 9) ELISA Kit
SKU:
AEES00345
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
