The Rat CK-MB (Creatine Kinase-MB Isoenzyme) CLIA Kit is specifically designed for the precise measurement of CK-MB levels in rat serum, plasma, and tissue homogenates. This kit offers unparalleled sensitivity and accuracy, ensuring dependable and consistent results for a variety of research purposes.CK-MB is a cardiac-specific biomarker that is released into the bloodstream following a heart attack or other cardiac conditions. Monitoring CK-MB levels can provide valuable insights into cardiac health and help diagnose and manage cardiovascular diseases. This kit is an invaluable tool for studying cardiac biomarkers and advancing research in the field of cardiovascular medicine.
Product Name:
Rat CK-MB (Creatine Kinase MB Isoenzyme) CLIA Kit
SKU:
RTES00620
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
7.5 pg/mL
Detection range:
12.5-800 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
