The Rat CNTF (Ciliary Neurotrophic Factor) ELISA Kit is a specialized assay designed for the quantitative measurement of CNTF levels in rat biological samples. CNTF is a neurotrophic factor known for its essential role in the survival and differentiation of neuronal cells in the peripheral and central nervous systems. This ELISA kit allows researchers to accurately quantify CNTF levels, providing valuable insights into neurodevelopment, neural repair, and neuroprotection mechanisms. By detecting and measuring CNTF levels, researchers can gain a deeper understanding of its functions in neuronal growth, synaptic plasticity, and neuroprotection. CNTF has been implicated in various neurological conditions, making accurate measurement crucial for studying its involvement in neurodegenerative diseases, neural injury, and other nervous system disorders. The Rat CNTF ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. With stringent quality control standards in place, this kit delivers reliable performance and ease of use, making it a powerful tool for research focused on unraveling the neuroprotective and neuroregenerative properties of Ciliary Neurotrophic Factor.
Product Name:
Rat CNTF (Ciliary Neurotrophic Factor) ELISA Kit
SKU:
AEES00351
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
23.44 pg/mL
Detection range:
39.06-2500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
