Rat CRH (Corticotropin Releasing Hormone) ELISA Kit
The Rat CRH (Corticotropin Releasing Hormone) ELISA Kit is a state-of-the-art assay system developed to precisely quantify levels of CRH in various rat biological samples. Corticotropin Releasing Hormone serves as a critical mediator of the body's stress response, controlling the release of hormones that affect functions such as metabolism, immune response, and behavior. By using this ELISA kit, researchers can accurately measure CRH levels, facilitating a deeper understanding of the body's stress regulation mechanisms and their impact on physiological processes and adaptations. CRH plays a pivotal role in orchestrating the body's response to stress, influencing adrenal gland function and the production of stress-related hormones like cortisol. Through the precise measurement of CRH using this ELISA kit, researchers can gain valuable insights into the intricate pathways involved in stress regulation, neuroendocrine responses, and the broader physiological effects triggered by stress-related stimuli. The Rat CRH (Corticotropin Releasing Hormone) ELISA Kit offered by Assay Genie delivers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under rigorous quality control standards, this kit provides robust performance and user-friendly protocols, making it an excellent choice for researchers investigating stress responses, neuroendocrine pathways, and related physiological processes.
Product Name:
Rat CRH (Corticotropin Releasing Hormone) ELISA Kit
SKU:
AEES00369
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
