The Rat CRP (C-Reactive Protein) ELISA Kit is a specialized assay meticulously crafted for the precise quantitative measurement of C-Reactive Protein levels in various biological samples. C-Reactive Protein is a crucial biomarker of inflammation and is synthesized by the liver in response to systemic inflammation, infection, or tissue injury. Elevated levels of CRP have been associated with various inflammatory conditions, cardiovascular diseases, and infections, making it an indispensable marker for assessing acute-phase responses and predicting disease prognosis. Accurate quantification of CRP is essential for understanding the inflammatory status of an individual and its significance in various pathophysiological processes. The Rat CRP ELISA Kit offered by Assay Genie provides exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control measures, this kit delivers robust performance and user-friendly protocols, making it an excellent choice for cutting-edge research and diagnostic applications.
Product Name:
Rat CRP (C-Reactive Protein) ELISA Kit
SKU:
AEES00436
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
