The Rat CXCR3 (CXC Chemokine Receptor 3) CLIA Kit is a powerful tool for researchers looking to accurately measure levels of CXCR3 in rat samples. This kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.CXCR3 is a key chemokine receptor involved in immune response and inflammation, playing a crucial role in various diseases such as autoimmune disorders, cancer, and infectious diseases.
By measuring CXCR3 levels, researchers can gain valuable insights into disease progression and potential therapeutic targets.With its easy-to-use format and robust performance, the Rat CXCR3 CLIA Kit is an essential tool for any researcher studying the role of CXCR3 in health and disease. Order yours today from Assay Genie to accelerate your research efforts.
Product Name:
Rat CXCR3 (CXC-Chemokine Receptor 3) CLIA Kit
SKU:
RTES00159
Target:
Rat CXCR3 (CXC-Chemokine Receptor 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CXCR3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CXCR3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CXCR3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CXCR3. You can calculate the concentration of Rat CXCR3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
97-110
93-108
Average (%)
94
102
99
1:4
Range (%)
94-108
92-103
84-97
Average (%)
101
97
91
1:8
Range (%)
103-118
100-118
98-113
Average (%)
108
107
106
1:16
Range (%)
100-116
90-102
85-100
Average (%)
106
95
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
97
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
95-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.08
278.5
930.3
102.1
277.96
944.41
Standard deviation
10.08
21.89
84.29
11.61
29.44
103.41
C V (%)
10.6
7.86
9.06
11.37
10.59
10.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CXCR3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CXCR3 in samples. No significant cross-reactivity or interference between Rat CXCR3 and analogues was observed.
