Rat Protein C Receptor, Endothelial (PROCR) ELISA Kit
The Rat PROCR (Protein C Receptor, Endothelial) ELISA Kit is a powerful tool for detecting and measuring levels of the PROCR protein in rat samples. With high sensitivity and specificity, this kit delivers accurate and reliable results for research applications in the fields of angiogenesis, vascular biology, and disease-related studies.PROCR is an important receptor protein that plays a key role in regulating blood coagulation, inflammation, and cell signaling processes. Its dysregulation has been linked to various diseases, including thrombosis, sepsis, and cancer, making it a valuable target for biomarker analysis and therapeutic development.
With easy-to-follow protocols and robust performance, the Rat PROCR ELISA Kit from Assay Genie is an essential tool for researchers looking to study the function and expression of the PROCR protein in rat models. Unlock new insights into vascular biology and disease mechanisms with this cutting-edge ELISA kit.
Product Name:
Rat Protein C Receptor, Endothelial (PROCR) ELISA Kit
Product Code:
RTDL00869
Size:
96 Assays
Target:
PROCR
Synonyms:
CD201, CCCA , CCD41, BA42O4.2, EPCR, Endothelial Protein C Receptor, Cell Cycle, Centrosome-Associated Protein, Activated Protein C Receptor
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
