The Rat FGF23 (Fibroblast Growth Factor 23) ELISA Kit is a specialized assay designed for the quantitative detection of FGF23 levels in various biological samples obtained from rat models. FGF23 is a key regulator of phosphate and vitamin D metabolism, playing a crucial role in maintaining mineral homeostasis and bone health. This ELISA kit provides researchers with a precise and reliable method to quantify FGF23 levels, allowing for a comprehensive understanding of its involvement in bone mineralization, renal function, and mineral metabolism. By accurately measuring FGF23 with exceptional sensitivity and specificity, researchers can advance their knowledge of FGF23's role in various physiological processes and pathological conditions, including chronic kidney disease and genetic disorders affecting mineral metabolism. Manufactured under stringent quality control standards, the Rat FGF23 ELISA Kit ensures robust performance, accurate results, and ease of use, making it an invaluable tool for investigations into the complex interplay of FGF23 in physiological and pathological pathways in rat models.
Product Name:
Rat FGF23 (Fibroblast Growth Factor 23) ELISA Kit
SKU:
AEES00545
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
