The Rat GAL3 (Galectin 3) ELISA Kit is a specialized assay designed for the precise and quantitative measurement of Galectin 3 levels in rat samples. Galectin 3 is a multifunctional protein involved in various biological processes, including inflammation, cell proliferation, differentiation, and apoptosis. Its role in modulating immune responses, cell adhesion, and signaling pathways underscores its significance in diverse physiological and pathological conditions. Accurate detection and quantification of Galectin 3 levels are crucial for investigating its involvement in disease mechanisms, particularly in conditions related to inflammation, fibrosis, cancer, and cardiovascular diseases. The Rat GAL3 ELISA Kit provides researchers with a reliable tool to explore the role of Galectin 3 in cellular processes and pathological conditions in rat models. With exceptional sensitivity and specificity, the Rat GAL3 ELISA Kit from Assay Genie ensures precise and reproducible results. Manufactured under strict quality control measures, this kit delivers reliable performance and user-friendly protocols, making it an excellent choice for research in the fields of immunology, inflammation, and disease pathology.
Product Name:
Rat GAL3 (Galectin 3) ELISA Kit
SKU:
AEES00403
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
